Evaluation of Anti-Depressant Effect of Banana Pulp on Mice

Sana Sarfaraz1*, Manal Khan2, Tanveer Bano2, Saba Aftab2, Sajida Duaa Shakeel2, Ulfat Akhtar2 and Reem Fatima2

1Department of Pharmacology, Faculty of Pharmacy & Pharmaceutical Sciences, University of Karachi-Karachi, Pakistan

2Faculty of Pharmacy, Jinnah University for Women Karachi-74600, Pakistan

*Corresponding author: Sana Sarfaraz, Department of Pharmacology, Faculty of Pharmacy & Pharmaceutical Sciences, University of Karachi-Karachi, Pakistan. E-mail: sana.sarfraz@live.com

Citation: Sarfaraz S, Manal Khan, Tanveer Bano, Saba Aftab, et al.(2020) Evaluation of anti-depressant effect of banana pulp on mice. Int J Pharm Sci Clin Pharm 1(1): 6-9.


Received Date: May 28, 2020; Accepted Date: June 30, 2020; Published Date: July 02, 2020


Background: The prevalence of depression is alarming all over the world afflicting billions of individuals. Depression is a common mental disorder and is one of the major causes of morbidity worldwide. Although, there are known effective treatment for depression, but antidepressant therapy usually causes a wide range of undesirable adverse effects and also increases the risk for suicide in individuals with ongoing treatment. So in order to reduce the risks associated, a study was conducted to evaluate the antidepressant potential of Musa paradisiacal Linn, commonly known as Banana fruit and to assess whether the natural source could be used to treat depression.

Methodology: Banana pulp was administered to the mice(n=6) i.e. 0.3ml calculated according to weight and given daily for one month and compared with control group of mice. To evaluate the activity, forced swim test was performed.

Results: The results showed highly significant anti-depressant effect at day 14 which was then gradually reduced at day 21.

Conclusion: It was concluded from the results that banana pulp has an antidepressant effect, as it is a rich source of neurotransmitter like serotonin, catecholamine and its precursor amino acid and tryptophan, which provides mood and energy boosting condition. It could be recommended as a non-pharmacological diet in individuals suffering from depression as it is cheap and easily available.

Keywords: Depression; Forced Swim Test; Musa pulp; Selective serotonin reuptake inhibitors;


Depression is a state of dejection, gloom, sadness. Major depressive disorder is a serious and very common medical illness. It is a self-directed hatred, feeling of helplessness, negative feelings like disappointment, weakness, frustration, grief and sorrow or situation in which one accuses oneself for being helpless [1].

The symptoms of depression affect the daily activities or the way person reacts. It can cause multiple physical and emotional problems that can result in low state of functional activity. Clinical depression is a diverse term that cannot be defined in nosological class [1-2].

Maniac Depression is also known as bipolar depression having serious swings in mood, behavior, thinking and energy to the extreme level resulting in decreased activity it may last from days to months. Psychotic Depression is a depressive psychosis that causes severe illness of depression with some form of psychosis like delusions, hallucinations with a depressive theme such as delusion of poverty, sickness, and regrets [3].

Dysthymia is also known as persistent depressive disorder as it remains for at least two years having less severe symptoms but lasts for minimum two years [4]. Perinatal Depression is encountered by the women after giving birth or during pregnancy. It often includes episodes of insomnia, tiredness, and weight gain and mood changes.

Seasonal Disorder appears every year in the winter season. Winter depression associated with increased sleep, social withdrawal and weight gain. About one among 15 individuals encounter depression in one year and 1 in 6 individual affected at some stages in their life [5].

Numbers of factors that can cause depression include emotional abuse, death or loss, genetic, certain medications, conflicts with family members or can be due to serious illness from other medical condition [6].

According to the study people prefer non pharmacological treatment to overcome depression which results in positive mental health and able to perform their usual functioning. Many effective non pharmacological activities have been approved to lower the effects of depression which include interpersonal psychotherapy, physical activity programs, light therapy, behavioral activation and healthy food that decreases the symptoms of depression and causes feeling of euphoria like walnuts, berries, onions, mushrooms, dark green leaf, tomatoes.

Banana is the common name of fruit belonging to family Musaceae and genus Musa. It is an energy booster, rich source of vitamins, minerals and fibers and considered to be a chief food. As it is a rich source of neurotransmitter like serotonin, catecholamine and its precursor amino acid and tryptophan, it provides mood and energy boosting effect. Banana is helpful in treating blood pressure especially as it is rich in potassium. Traditionally it was consumed in gastric irritations, constipation, and other digestive problems. Banana also gives a good effect in diabetic patients and improves electrolyte imbalance. It is a rich source for vitamin B which is helpful in production of new cells [7-8]. In our study we have evaluated the antidepressant activity of banana pulp, according to the previous study in which some of its nutrients were found helpful against the depressive symptoms [9].


Banana Pulp Extraction

The bananas were purchased from the local market in Karachi, Pakistan and were identified by Department of Pharmacognosy, Faculty of Pharmacy, Jinnah University for Women. The pulp of banana was obtained using mortar and pestle by crushing the banana, five to ten minutes before the administration of pulp so that it does not show black coloration.

Experimental Animal

Twelve Swiss Albino mice, either male or female were selected for the experiment weighing 16-28 grams, provided by the animal house of Jinnah University for Women. The mice were kept in plastic cages at least for a week prior to experimentation in order to make them used to the lab environment. The study was conducted after approval from the institution. The animals were kept at normal room temp. (25 ± 2º C) and fed with proper diet and water. The experimental animals were divided into two groups containing 6 mice in each (A and B) randomly. Group mice were labelled as control and Group B were administered banana pulp. The animals were handled as per the specification provided by Hubrecht and Kirkwood 2010 [10].


The study was conducted for a month. The resources required for the study were collected within 2 days approximately. The administration of banana pulp was done on daily basis for one month and results were noted every week. The mice in group B were given 0.3ml of banana pulp according to mice weight daily for a month. The mice in group A were administered 0.3ml distilled water.

Screening Test

Forced Swim Test: The antidepressant effect of banana pulp was checked using this test. The apparatus comprises of a cylindrical glass container in which water of specific temperature i.e. 20o-22oC is filled. The test was conducted once a week that is first reading was taken at Day 7th, 14 and last on day 21st after 40 minutes of administration of banana pulp. The mice were placed in the container individually and allowed to swim freely and using a stopwatch the time period of swimming was recorded for each mouse that is when the mouse stops swimming freely, it was taken out and time was recorded [11], (Figure-1).

Figure 1: Effect of banana pulp on Forced Swim Test

n=6, values are Mean ± S.D using Statistical analysis SPSS 21. Using one way Anova and Post hoc Tukey’s test applied according to which P values ***p≤0.001 is considered as significant as compared to control.

The data was analyzed using SPSS 21. Mean ± S.D (n=6) was used for presenting the data. Statistical analysis was performed using one-way Anova (analysis of variance) followed by Post hoc Tukey’s test.

Result and Discussion

An obligatory stress developed to rodents by Forced Swim Test (FST) which reflects a state of behavioral despair that is similar to human depression [12-13]. Animal model for preclinical screening of antidepressant are generally used [14]. Animals that are treated with antidepressants struggle more even in desperate situation and spend less time with immobility [15].

Forced swim test is a drug screening test for antidepressants; it is able to recognize the activity of broad spectrum of clinically effective antidepressants. Forced swim test has a high degree of predictive validity to major class of antidepressants such as Tricyclic antidepressants (TCAs), Monoamine oxidase inhibitors (MAOIs), atypical antidepressant, Selective serotonin reuptake inhibitors (SSRIs) and electroconvulsive therapy [16].

Banana has proven to give many beneficial effects like antidepressant effect, improve electrolyte balance. It is a rich source for Vitamin B. Banana belonging to family Musaceae has been used since many centuries for its non-pharmacological activity [17].

Banana is a rich source of neurotransmitters like serotonin, catecholamine which helps in energy boosting and mood boosting effect by elevating low levels of tryptophan [17].

According to the experimental design, forced swim test was performed on equal numbers of mice belonging to two groups’ i.e. Control and Treatment (Banana). Dose of 0.3ml was administered to each group of mice on daily basis while the effect was observed on Day 7th, 14 and 21st.

According to the results, the treatment group shows positive results. At Day 7 when readings were taken the struggling time of control group was higher as compared to treated group but as the day passed the struggling time of treatment was increased as compared to the control group on Day 14 and Day 21 which shows the positive effect of banana in treating Depression.

Constrained swim test isn’t just a marker of energizer impact of medications however it is additionally utilized as a pointer of dejection in rodents [18].

Depression is clinically defined as complex of mental and neuroendocrine effects. It is a widely prevailing neurological disorder in modern paced life. Stressful lifestyle promotes the progression of depressive disorder as the stress can significantly effects the function of central nervous system by modifying a number of neurotransmitters, endocrine and neuroendocrine systems [19-21].

Tryptophan is the sole precursor of peripheral and centrally produced serotonin. Tryptophan and 5 Hydroxytryptophan (5-HTP) are natural alternatives to conventional antidepressants used in the treatment of unipolar depression and dysthymia [22].

Several studies have shown that people with depression may have lower than normal tryptophan levels [23].

The consumption of a diet or solution that contains large number of neutral amino acids but lacks tryptophan, results in an immediate and effective reduction of plasma tryptophan. The fact that dietary removal of tryptophan results in protein synthesis in the liver which uses the tryptophan available in the plasma and thus lowers its level [24].

Large number of dietary neutral amino acids in addition to increase hepatic protein synthesis, compete with tryptophan for transportation across the blood brain barrier thus limiting the entry of tryptophan into the brain, resulting in decreased levels of centrally acting tryptophan and thus serotonin.

The above outcomes demonstrate increased struggling time which is like same impact delivered by standard medication. In contempt of the extensive use of synthetic drugs to treat depression which often correlate with adverse effects and limitations. Research for a natural product with fast onset of action, wide safety margin and less side effects is now under consideration [25].


From our study it is concluded that banana pulp possess an anti-depressant activity. Since it is very commonly consumed and preferred in all populations as a useful nutrient. It can also be recommended to counteract stressful life style and depressive episodes occurring at different phases of our life by virtue of its antioxidant, MAO inhibitory and pro-serotonergic and pro-adrenergic activities. Additional benefit being that banana fruit is available everywhere and considered safer and cheaper than antidepressant medicines. Future studies should target the exact molecular mechanism by which this effect is observed.


  1. Wollman Benjamin. Depression: The Psychosocial Therapy. USA: 1990 by John Wiley and Sons, Inc. 2015;6-7.
  2. Brody DJ, Pratt LA, Hughes JP. Prevalence of depression among adults aged 20 and over: United States, 2013-2016. US Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Health Statistics. 2018.
  3. Alharbi R, Alsuhaibani K, Almarshad A, Alyahya A. Depression and anxiety among high school student at Qassim Region. Journal of family medicine and primary care. 2019;8(2):504-510.
  4. Lin LY, Sidani JE, Shensa A, Radovic A, Miller E, Colditz JB and et al. Association between social media use and depression among US young adults. Depression and anxiety. 2016;33(4):323-331.
  5. Sana Sarfaraz, Urooba Iqbal, Rabia Khalid, Nameera Ahmed, Anum Aslam, Bushra Rubab. Anti-depressant effects of sunlight and awareness regarding light therapy. WJPPS. 2016;5(7):154-162.
  6. Manji, SK, Drevetsm WC and Charneym DS. The cellular neurobiology of depression. Nat. Me. 2001;7(5):541-547
  7. Pereira A, Maraschin M. Banana (Musa spp) from peel to pulp: Ethnopharmacology, source of bioactive compounds and its relevance for human health. J Ethnopharmacol. 2015;160:149–163.
  8. Davey MW, Van Den Bergh I, Markham R, Swennen R, and Keulemans J. Genetic variability in Musa fruit pro vitamin A carotenoids, lutein and mineral micronutrient contents. Food Chemistry. 2009;115(3):806-813.
  9. Tee TP, Hassan H. Antidepressant-like activity of banana peel extract in mice. American Medical Journal. 2011;2(2):59-64.
  10. Hubrecht R and Kirkwood J. The UFAW Handbook on Care and Management of Laboratory and Other Research Animals. 8th edition. Oxford: Wiley Blackwell. 2010.
  11. Lucki I. The forced swimming test as a model for core and component behavioral effects of antidepressant drugs. Behav Pharmacol. 1997;8:523–532.
  12. Khan H, Perviz S, Sureda A, Nabavi SM, Tejada S. Current standing of plant derived flavonoids as an antidepressant. Food and Chemical Toxicology. 2018;119:176-88.
  13. Roni Yankelevitch-Yahav, Motty Franko, Avrham Huly, and Ravid Doron. The Forced Swim Test as a Model of Depressive-like Behavior. J Vis Exp. 2015;(97):52587.
  14. Takao,Keizo and Miyakawa, Tsuyoshi. Light/dark Transition Test for Mice. Journal of Visualized Experiments. 2016;1:e104.
  15. Hazarika I. Anti-Depressant Activity of Aqueous Leaf Extract of Pergularia daemia in In-Vivo Animal Models. Research & Reviews: Journal of Neuroscience. 2019;5(3):21-6.
  16. Sarfaraz S, Najam RA, Azhar I, Riaz B, Anser HU. Anxiolytic and CNS depressant Effects of ethanolic extract of Cleome brachycarpa revealed after Neuropharma-cological Screening. W JPS. 2014;2(17):605-610.
  17. Miland Parle and Suman Malik. Anti-Depressant Potential of Banana Fruit. International Journal of Medical Sciences. 2010;2(2): 148-151.
  18. Cryan JF, Mombereau C and Vassout A. The tail suspension test as a model for assessing antidepressant activity: Review of pharmacological and genetic studies in mice. Neurosci. Biobehav. Rev. 2005;29:571-625.
  19. Mok RY, Fong SY, Yang M, Sung TH, Wong YC, Ren T, et al. Effect of herbal and nutritional products on the central nervous system effects of haloperidol: a systematic review. Journal of Asian Association of Schools of Pharmacy. 2019;8:22-49.
  20. Elkhayat ES, Alorainy MS, El-Ashmawy IM, Fathi S. Potential antidepressant constituents of Nigella sativa seeds. Pharmacognosy magazine. 2016;12(Suppl 1):S27-S31.
  21. Taya Varteresianand Helen Lavretsky. Natural Products and Supplements for Geriatric Depression and Cognitive Disorders: An Evaluation of the Research. Curr Psychiatry Rep. 2014;16(8):456.
  22. Trisha A Jenkins, Jason CD Nguyen, Kate E Polglaze and Paul P. Bertrand. Influence of Tryptophan and Serotonin on Mood and Cognition with a Possible Role of the Gut-Brain Axis. Nutrients. 2016;8(1):56.
  23. Glenda Lindseth, Brian Helland and Julie Caspers. The Effects of Dietary Tryptophan on Affective Disorders. Arch Psychiatr Nurs. 2015;29(2):102–107.
  24. Neumeister A. Tryptophan depletion, serotonin, and depression: where do we stand? Psychopharmacology Bulletin. 2003;37(4):99-115.
  25. Samad N, Muneer A, Ullah N, Zaman A, Ayaz MM, Ahmad I. Banana fruit pulp and peel involved in antianxiety and antidepressant effects while invigorate memory performance in male mice: Possible role of potential antioxidants. Pak J Pharm Sci. 2017;30(3(Suppl.)):989-995.